A new RP-HPLC method for the determination of Tenofovir Disoproxil Fumarate in pure form and pharmaceutical formulation

  • I. Manojkumar Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024.
  • D. Saravanan Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024 https://orcid.org/0000-0002-6763-9840
  • A. Maheswaran Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024.
  • P. Divakar Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024.
Keywords: Tenofovir, Estimation, Analytical method, HPLC

Abstract

The present study aimed to develop the new method for the estimation and validation of tenofovir in pure form and in pharmaceutical dosage form by RP-HPLC. The chromatogram of tenofovir was developed through column (Hyper ODS2 C18), UV detection at 260 nm at a flow rate of 1.2 ml/min with Methanol and Phosphate buffer (90:10) as mobile phase. The method was validated by various validation parameters such as accuracy, precision, linearity, specificity as per the ICH guidelines. A linearity range and retention time of Tenofovir were found to be 20-110 µg/ml and 2.1 min respectively. The % RSD of the Tenofovir was found to be 0.7. The % recovery was obtained as 99.7% for standard and 96.32% for tablets. This method was simple, accurate, precise and sensitive. Hence, the developed method was employed for the routine analysis of Tenofovir in the pharmaceutical dosage form.

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Published
13-10-2020
How to Cite
I. Manojkumar, D. Saravanan, A. Maheswaran, & P. Divakar. (2020). A new RP-HPLC method for the determination of Tenofovir Disoproxil Fumarate in pure form and pharmaceutical formulation. International Journal of Research in Pharmaceutical Sciences and Technology, 2(1), 17-24. https://doi.org/10.33974/ijrpst.v2i1.233
Section
Research Article