International Journal of Research in Pharmaceutical Sciences and Technology https://rubatosis.org/journals/index.php/ijrpst <p align="justify">Rubatosis Publication has launched its first online peer review scientific journal named International Journal of Research in Pharmaceutical Sciences and Technology (<strong>IJRPST, ISSN Online: 2581-9143</strong>). IJRPST will be published quarterly per year in January, April, July, and October. The journal publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences and Technology.</p> Rubatosis Publications en-US International Journal of Research in Pharmaceutical Sciences and Technology 2581-9143 Reconciling of Analytical method development and Validation by Utilization of HPLC for Novel Polymer and Lipid based Drug Delivery Systems https://rubatosis.org/journals/index.php/ijrpst/article/view/278 <p>Using polymer and lipid-based drug delivery techniques, the primary objective is to enhance bioavailability, solubility, and dissolution. Studies have proven therapeutic effectiveness, stability, toxicity, and illness detection. This research aims to show how HPLC may be used to measure novel polymer and lipid-based drug delivery systems. As explained in this article, HPLC is one of the most widely used techniques for assessing drug concentration in lipid and polymer formulations under a range of physical conditions. Because HPLC may be used with some detectors and columns, it might be challenging for researchers to find the ideal conditions to develop a simple, sensitive, and repeatable process for their lipid and polymer matrices study. The goal of this review paper is to provide researchers with information on a range of HPLC techniques used in lipid and polymer drug delivery systems.</p> Nayela Ghazal Abdul Waheed Copyright (c) 2021 Nayela Ghazal, Abdul Waheed https://creativecommons.org/licenses/by-nc-sa/4.0 2021-11-23 2021-11-23 2 4 ODFs: a multi-faceted aid to the formulator https://rubatosis.org/journals/index.php/ijrpst/article/view/265 <p>The first films introduced into market are around 1970, from then there are many new technologies are developed in preparation and also using polymers. From Natural to synthetic polymers each polymer has their advantages over one another. Various techniques in preparation of oral films such as solvent casting, semi-solid casting, hot-melt extrusion, solid dispersion extrusion and rolling to the modern techniques are developed from time to time. The most recent technique used is 3D printing which forms the films by forming layer on layer. In this literature along with polymers used, methods for preparing of films; approved drugs in orange books and present clinical trial studies were discussed.</p> Dr. Madhu Gudipati Copyright (c) 2021 Dr. Madhu Gudipati https://creativecommons.org/licenses/by-nc-sa/4.0 2021-10-25 2021-10-25 2 4 10.33974/ijrpst.v2i3.265 In Vitro Antiviral Activity of AegleMarmelos against Influenza A (H1N1) Pdm09 https://rubatosis.org/journals/index.php/ijrpst/article/view/271 <p>Due to the high prevalence of viral infections having no specific treatment and the constant appearance of resistant viral strains, the development of novel antiviral agents is essential. The aim of this study was to evaluate the antiviral activity of aqueous and ethanolic extracts of Aeglemarmelosagainst Influenza A (H1N1) pdm09. The extract was subjected to cytotoxicity as well as antiviral activity on MDCK cells. The concentration range from 10 to 100μg/ml in MDCK cell line was conducted. It showed that the treated cells with drug was nontoxic to the cells at the concentration of 50μg/ml for ethanolic and 60μg /ml for aqueous extract at 48 hrs to 72 hrs and was comparable to that of controls. Hence crude extract of both ethanolic and aqueous up to 50-60μg /ml (CC50) could be used to test the antiviral activity without affecting much of the cell viability. In simultaneous anti viral assay, 80% viral inhibition was observed at the concentration of 50μg/ml in aqueous extract whereas 100 % viral inhibition was observed in ethanolic extract at concentration of 60μg/ml. In the post treatment assay, the aqueous extract did not show viral inhibition, whereas the ethanolic extract showed 100% reduction at the concentrations of 60μg/ml. This data suggest that ethanolic extract inhibit the Influenza virus infection by blockage of viral attachment by inhibition of viral HA protein, this is consistent with previous studies.</p> S.G. Raman I. Sarath Chandiran Copyright (c) 2021 https://creativecommons.org/licenses/by-nc-sa/4.0 2021-06-30 2021-06-30 2 4 1 4 Antibacterial Activity of AegleMarmelos against Fruit Extracts https://rubatosis.org/journals/index.php/ijrpst/article/view/272 <p>The antibacterial activity of the ethanol, and aqueous extracts from the fruit of&nbsp;<em>A. marmelos</em>&nbsp;was studied using disc diffusion method against&nbsp; five pathogenic bacterial strains, three Gram-positive bacteria (<em>B. cereus, S. epidermidis, S. aureus</em>) and two Gram-negative bacteria (<em>E. aerogens, K. pneumoniae</em>).The maximum 14.3mm zone of inhibitionwas observed at the concentration of 40mg/ml foraqueous extract. This may be due to the presence of the compounds Cuminaldehyde and Eugenol. In the case of ethanol, the maximum antibacterial activity was seen against <em>E. aerogens</em>followed by <em>S. epidermidis</em>and <em>K. pneumonia.</em> At the highest concentration of 160 mg/ml, it showed 18.3mm zone of inhibition. The antibiotic susceptibility showed that among all the bacterial strains <em>S. aureus</em>was found more susceptible to ampicillin followed by <em>K. pneumonia</em>. Results suggest that the ethanolic extract has significant antibacterial activity against tested bacteria. The present study justifies the claimed uses of&nbsp;<em>A. marmelos</em>&nbsp;in the traditional system of medicine to treat various infectious diseases.</p> I. Sarath Chandiran S.G.Raman Copyright (c) 2021 https://creativecommons.org/licenses/by-nc-sa/4.0 2021-06-30 2021-06-30 2 4 5 8 10.33974/ijrpst.v2i4.272 Formulation and Evaluation of Loperamide HCl fast dissolving tablets using cross treated Xanthan Gum https://rubatosis.org/journals/index.php/ijrpst/article/view/277 <p>Various fast dissolving drug formulations have been recently introduced into the market. This work proposes the design of novel oral fast dissolving tablets (FDT’s) of loperamide HCl with special emphasis on disintegration and dissolution studies. The main goal was augmenting the adherence to treatment of diseases which happen with diarrhea in soldiers who are exposed to diverse kinds of hostile environments. The excipients were used for this study was based on the compatibility studies. Optimized orally oral fast dissolving tablets were prepared by the direct compression method from cross xanthan gum development to the industrial scale technique. The results show that loperamide HCl FDT’s &nbsp;offers a rapid beginning of action and improvement in the bioavailability of poorly absorbed drugs. The manufactured FDT’s complied with the pharmacopeia guidelines regarding hardness, weight variation, thickness, friability, drug content, wetting time, percentage of water absorption, disintegration time, and <em>in-vitro </em>dissolution profile. Drug compatibility with excipients was checked by FTIR studies.</p> K. Vinod Kumar S. Manohar Babu T. Srinivas I. Syamala B. Sirisha SK. Shadik Copyright (c) 2021 K. Vinod Kumar, S. Manohar Babu, T. Srinivas, I. Syamala, B. Sirisha , SK. Shadik https://creativecommons.org/licenses/by-nc-sa/4.0 2021-10-25 2021-10-25 2 4 Formulation and Evaluation of ethyl cellulose microspheres containing diclofenac sodium https://rubatosis.org/journals/index.php/ijrpst/article/view/276 <p>Diclofenac&nbsp;sodium belongs to a class of drugs known as non-steroidal anti-inflammatory drugs (NSAIDs). This medication&nbsp;is used to relieve&nbsp;joint pain&nbsp;from&nbsp;arthritis. It has shorter half life (2hr). It undergoes extensive first pass metabolism. Frequent dosing is required in case of conventional dosage form. The objective of present study was to develop ethyl cellulose-based sustained release diclofenac sodium microspheres to reduce the dosing frequency to achieve targeted drug delivery system. The diclofenac sodium-loaded ethyl cellulose microspheres were formulated by emulsification technique and the physicochemical properties of the formulations were characterized. Compatibility studies were carried out using FTIR studies. The percentage drug content was studied in three different pH media (pH 1.2, pH 6.8 and pH 8.0). The entrapment efficiency of these microspheres was between 36% and 80.95%. The obtained microspheres showed good flow properties, which were evaluated in terms of angle of repose (15.27 – 20.30), bulk and tapped densities (0.34-0.39 and 0.37-0.48, respectively), Carr indices and Hausner ratio (20-45 and 1.03-1.80, respectively). Particle size and percentage drug content depended on the nature and content of polymer used. In formulation (F) first drug and ethyl cellulose were use in 1:1, 1:2 and 1:3 ratios. As the concentration of polymer increases the drug encapsulation in microsphere were increasing. Formulation F3 was found best formulation. Thus, overall study reveals that prepared microspheres of diclofenac sodium by using ethyl cellulose using binary organic mixture as solvent at different ratios to enhances the percentage drug content and flow properties.</p> Srinivas Thota B. Kusuma M. Rarevati P.Narendra S. Manohar Babu Copyright (c) 2021 Srinivas Thota, B. Kusuma, M. Rarevati, P.Narendra , S. Manohar Babu https://creativecommons.org/licenses/by-nc-sa/4.0 2021-10-25 2021-10-25 2 4