A new RP-HPLC method for the determination of Tenofovir Disoproxil Fumarate in pure form and pharmaceutical formulation

Authors

  • I. Manojkumar Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024. Author
  • D. Saravanan Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024 Author
  • A. Maheswaran Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024. Author
  • P. Divakar Department of Pharmaceutical Analysis, Jaya College of Paramedical Sciences, College of Pharmacy, Chennai, Tamil Nadu, India-602024. Author

DOI:

https://doi.org/10.33974/ijrpst.v2i1.233

Abstract

The present study aimed to develop the new method for the estimation and validation of tenofovir in pure form and in pharmaceutical dosage form by RP-HPLC. The chromatogram of tenofovir was developed through column (Hyper ODS2 C18), UV detection at 260 nm at a flow rate of 1.2 ml/min with Methanol and Phosphate buffer (90:10) as mobile phase. The method was validated by various validation parameters such as accuracy, precision, linearity, specificity as per the ICH guidelines. A linearity range and retention time of Tenofovir were found to be 20-110 µg/ml and 2.1 min respectively. The % RSD of the Tenofovir was found to be 0.7. The % recovery was obtained as 99.7% for standard and 96.32% for tablets. This method was simple, accurate, precise and sensitive. Hence, the developed method was employed for the routine analysis of Tenofovir in the pharmaceutical dosage form.

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UV spectrum of Tenofovir

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Published

25-09-2020

Issue

Vol. 2 No. 1 (2020): Volume 2 Issue 1

Section

Research Article

License

Copyright (c) 2020 I. Manojkumar, D. Saravanan, A. Maheswaran, P. Divakar (Author)

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How to Cite

A new RP-HPLC method for the determination of Tenofovir Disoproxil Fumarate in pure form and pharmaceutical formulation. (2020). International Journal of Research in Pharmaceutical Sciences and Technology, 2(1), 17-24. https://doi.org/10.33974/ijrpst.v2i1.233